Figure 1
From: Rapid and efficient genetic manipulation of gyrencephalic carnivores using in utero electroporation
Procedure for in utero electroporation in ferrets. (A) A pregnant ferret was anesthetized at E36, and the right uterus was exposed. (B) The exposed uterus was kept wet with PBS by using a syringe (arrowhead). Note that the embryo within the uterus was invisible under epi-illumination. (C) Visualizing ferret embryos in the uterus using transmitted light derived from an optical fiber cable (arrowhead). Performing in utero electroporation in a dark room is helpful for visualizing ferret embryos in the uterus. (D) A ferret embryo in the uterus revealed with transmitted light. The pigmented iris and injected plasmid solution containing Fast Green were visible (arrow and arrowhead, respectively). Scale bars, 2 cm.