Figure 2

GFP signals in the ferret cerebral cortex induced by in utero electroporation. pCAG-GFP was introduced to the ferret cerebral cortex by using in utero electroporation (50 V, 50 ms) at E40, and ferret embryos were dissected 2 days later. Macroscopic dorsal views of the ferret brain (B) and its GFP fluorescence (A) are shown. Note that GFP signals were visible on the brain surface (arrowhead). a, anterior; p, posterior. Lower (C) and higher (D) magnification images of the coronal section of the electroporated brain. GFP-positive cells extending their processes toward the pial surface were observed in the cerebral cortex (arrowheads). Asterisks, the lateral ventricle. Scale bars, 2 mm (B), 1 mm (C), 200 μm (D).