Figure 5

In utero electroporation performed at an earlier time point during development. In utero electroporation was performed at E31 to express GFP in the ferret cerebral cortex, and the brain was dissected at P0. Macroscopic dorsal views of the ferret brain (B) and its GFP fluorescence (A, arrowhead) are shown. a, anterior; p, posterior. Low (C) and high (D) magnification images of GFP fluorescence in the cerebral cortex in the coronal section. The area within the white box is magnified and shown in (D). Note that numerous GFP-positive neurons are visible in the cortical plate even without GFP staining (arrowheads). Asterisk, the lateral ventricle. CP, cortical plate; IZ, intermediate zone. Scale bars, 2 mm (B), 1 mm (C) and 200 μm (D).