Figure 7

Double labeling using in utero electroporation. Cortical neurons were transfected with pCAG-GFP and pCAG mCherry using in utero electroporation at E33. The brain was dissected out at P20, and coronal sections were made. (A) GFP signals and mCherry signals were clearly observed on the brain. (B) Numerous GFP-positive neurons were found in deep cortical layers. GFP-positive axons were also clearly visible even without GFP immunostaining (arrowhead). The area within the white box is magnified and shown in (C). Note that most GFP-positive neurons were also positive for mCherry (C). The morphology of GFP-positive neurons was clearly visible even without GFP immunostaining. Scale bars, 5 mm (A), 1 mm (B) and 200 μm .(C).