Formic acid treatment reveals an increase in small 1C2+inclusions in the Hdh140Q/3xFlag20Qstriatum in comparison to Hdh140Q/3xFlag7Qstriatum. (A) Images of the striatum from 6 month-old and 2 year-old Hdh 140Q/3xFlag7Q and Hdh 140Q/3xFlag20Q mice probed with 1C2 antibody (red) following formic acid treatment. Nuclei were visualized with To-Pro-3. Examples of “aggregate chains” are indicated with white arrows. Scale bar = 25 μm. (B) Quantification of the total 1C2+ pixel area per microscopic field in the striatum of Hdh 140Q/3xFlag7Q and Hdh 140Q/3xFlag20Q mice at 6 months and 24 months of age (n=3 mice for each age and genotype, mean ± SEM). * P <0.05.