Time course and quantification of htt inclusions in the Hdh140Q/3xFlag7Qand Hdh140Q/3xFlag20Qstriatum. (A) Representative confocal images of the striatum at 2 months, 4 months, 6 months, and 24 months of age. An image from a 24 month-old wild-type (WT) control is also shown for comparison. 3xFlag7Q- and 3xFlag20Q-htt were detected with the FLAG M2 antibody (green), while htt inclusions were visualized using the MW8 antibody (red). Nuclei were visualized with To-Pro-3 (blue). White arrows indicate inclusions co-staining with the Flag antibody. Scale bar = 25 μm. (B) Quantification of total htt aggregate number/imaging field. (C) Quantification of total htt aggregate number (T), nuclear aggregate number (N) and neuropil aggregate number per imaging field at 6 months and 24 months of age (n=3 mice for each age and genotype). A significant difference in aggregate number between the Hdh 140Q/3xFlag7Q and Hdh 140Q/3xFlag20Q striatum was observed in the nuclear compartment at 6 months of age. Error bars=SEM, * P <0.05.