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Figure 3 | Molecular Brain

Figure 3

From: Neuropeptide complexity in the crustacean central olfactory pathway: immunolocalization of A-type allatostatins and RFamide-like peptides in the brain of a terrestrial hermit crab

Figure 3

Details of the median protocerebrum (mPc), the deutocerebrum (Dc), and the tritocerebrum (Tc), conventional fluorescence with Apotome structured illumination (A), and confocal laser scanning microscopy (B-H). A low power overview triple labeled for ASTir (green), synapsin (SYN; red) and the nuclear marker (blue). B-F: a dorsal (B) to ventral (F) series of confocal optical sections showing ASTir (black-white inverted images). G, H higher magnifications of the median protocerebrum with central complex. G shows a double labeling against ASTir and RFir. H shows the allatostatin channel only (image is black/white inverted). The arrowheads indicate single ASTir neurons, asterisks point the processes of allatostatin immunoreactive local interneurons. Abbreviations: 9 cell cluster (9), AMPN anterior medial protocerebral neuropil, AnN antenna II neuropil, CA cerebral artery, CB central body, LAN lateral antenna I neuropil, MAN median antenna I neuropil, PB protocerebral bridge, OGT olfactory globular tract, PMPN posterior median protocerebral neuropil, x chiasm of the olfactory globular tract.

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