Figure 4

Details of ASTir in the olfactory lobes (OL). A, B, D-F show conventional fluorescence with Apotome structured illumination, C, G, H are images obtained by confocal laser scanning microscopy. A overview of a section triple labeled for ASTir (green), synapsin (red) and the nuclear marker (blue). The boxed area in A is shown in a higher magnification on B and displays a group of strongly immunoreactive somata of neurons within the cell cluster (9). C higher magnification of ASTir local olfactory interneurons in cluster (9). D, E, F: higher magnification of tangential section of olfactory lobes in different perspective, double labeled for allatostatin (green) and nuclear counter stain (blue), showing strong ASTir in all olfactory glomeruli. G: a higher magnification of the olfactory glomeruli (OG) shows that the ASTir is strongly concentrated in the periphery of the glomeruli whereas medium staining intensity is visible in the central core. H1, H2, and inset: longitudinal sections of the olfactory glomeruli show that ASTir is concentrated in the subcap (Sc) and base (Ba) region of the glomeruli. The subcap of neighboring glomeruli is connected by ASTir fibres. H1 and the higher magnification in the inset display double staining for A-type allatostatin (green) and synapsin (red), H2 shows the allatostatin channel only (image is black/white inverted). Abbreviations: 10 cell cluster (10), Ca cap, Ba Base, OG olfactory glomeruli, OL olfactory lobe, Sc subcap.