Exposure of HgCl
compromised cytoskeleton components, mainly the β-tubulin, in cortical neurons. Cells were first cultured for 4 to 10 days and allowed to develop neurite and networks and were then exposed to 25 μM of HgCl2 for up to 24 hrs. Cultures were subsequently fixed and stained with antibodies against β-tubulin and rhodamine-phalloidin (A-D). E &F are the merged confocal images of β-tubulin and F-actin staining. The fluorescence intensity of β-tubulin, and not F-actin was reduced in neurons that were treated with 25 μM of HgCl2. Scale bar, 25 μm.