Skip to main content
Figure 4 | Molecular Brain

Figure 4

From: Columnar distribution of activity dependent gabaergic depolarization in sensorimotor cortical neurons

Figure 4

Single-cell and population-scale 2-photon Ca2+imaging in Fura-2 AM stained cortical slices. A: Two representative Fura-2 AM stained upper-layer pyramidal neurons imaged for 80 s starting 20 s after L6/WM electrical stimulation: (i) in normal ACSF (left panel); and (ii) in ACSF containing GABAA agonist isoguvacine (right panel). B: Time-course of stimulation-induced increase of [Ca2+]i for each of the 8 upper-layer pyramidal neurons imaged in normal ACSF (left), and in the presence of 10 μM isoguvacine (right). Vertical bar at right indicates color coding of normalized magnitude of stimulus-evoked increase of fluorescence (ΔF/F). C: Time-averaged post-stimulation increase of [Ca2+]i for each of the 8 neurons which provided the data shown in B. Fura-2 AM signal was averaged over 40 s beginning 20 s after the end of stimulation. D: Representative image for indication of ROI (1 to 8) of measuring Ca2+ signal across layer 2–3 in cortical slice. White arrow: location of electrical stimulation. Stimulus induced individual trace of 1 to 8 ROI before (black traces) and with application of isoguvacine (red traces) was shown in lower panel of C. Numbers in right side indicates the distance (μm) from stimulation. E: Average across-slice (n = 4) Ca2+ signal in layers 2–3 evoked by L6/WM stimulation across 1.2 mm slice sector in the presence (gray squares) or absence (black circles) of isoguvacine. In each of the 4 slices, the 1.2 mm slice sector was divided into 8x250 μm rectangular compartments. Error bars = ±1SE. F: Bar plot summarizing the Ca2+ imaging observations obtained under the following conditions. Numbers in bars indicate number of observations obtained under each condition; ** indicates statistical significance of difference between bracketed values is <0.001; NS indicates difference between bracketed values is not significant.

Back to article page