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Figure 1 | Molecular Brain

Figure 1

From: Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

Figure 1

Age-dependent formation of αS-immunopositive axonal swellings (αS-globules) in brains of αS tg mice . (a) Hematoxylin and eosin staining (upper two panels) showed no apparent changes in brains of αS tg mice (right) and non-tg littermates (left). Immunohistochemistry of αS (lower two panels) showed formation of αS-globules (arrows) in αS tg mice (right), but not in wild type littermates (left). Representative images for the thalamus are shown. Scale bar = 20 μm. (b) Age-dependent formation of αS-globules in the αS tg brain detected by anti-αS antibody. The numbers of globules with diameters >4 μm were unbiasedly counted in the striatum of αS tg brains at three ages: young (n = 5, 3–4 mo), adult (n = 3, 10–13 mo), and old (n = 3, 21–25 mo). (c) Double immunofluorescence was performed using αS as a globule identification. Kinesin heavy chain (kinesin hc) was positive (upper three panels) and neurofilament-light (NF-L) was negative (lower three panels). Representative images are shown for the pontine nuclei (upper) and striatum (lower). Scale bar = 5 μm.

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