Figure 2

Ultrastructure of αS-globules in brains of αS tg mice . Immunoelectron microscopic analysis was performed using anti-αS. αS-immunopositive globules (a) were characterized by lysosomal pathologies such as an αS-immunopositive multivesicular body (b: arrow), autophagic vacuoles (c), myelinosome (d: arrow), a myelinoid membrane (e: arrow), and a light multivesicular body (e: arrow head). Formation of a fingerprint profile (j) adjacent to a lipid droplet (j: L) and curvilinear bodies (f, h) are reminiscent of lysosome storage disease. Accumulation of mitochondria was also occasionally observed (f, i; blue). Some mitochondria were swollen and deformed (g). The globules are representatives of those in the thalamus (a-h) and striatum (i, j). The boxed area in panels with lower magnifications (a, c, f, and i) were enlarged in b, and j or in two figures (d, e, g, h). Scale bar = 2 μm for c, f, i; 200 nm for b, d, e, g, h, j.