Antigen | Host | Dilution | Mono/polyclonal | Source | |
---|---|---|---|---|---|
Primary antisera and antibodies | |||||
1 | α-synuclein | mouse | 1:500 (for EM) | monoclonal | BD Biosciences (610787) |
1:1000 (for GABA FIHC) | |||||
1:2000 | |||||
2 | α-synuclein | rabbit | 1:50 | polyclonal | Cell Signaling (#2628) |
3 | phosphorylated α-synuclein | mouse | 1:2000 | monoclonal | WAKO (pSyn#64) |
4 | nitrated α-synuclein | mouse | 1:50 | monoclonal | Santa Cruz Biotechnology (sc-32279) |
5 | 4-hydroxy-2-nonenal | mouse | 1:40 | monoclonal | NOF Corporation (MHN-020P) |
6 | Calbindin D-28 k | rabbit | 1:5000 | polyclonal | Swant (CB38) |
7 | Parvalbumin | mouse | 1:2000 | monoclonal | Merck Millipore (MAB1572) |
8 | Calretinin | rabbit | 1:2000 | polyclonal | Swant (7699/4) |
9 | Glutamic acid decarboxylase | rabbit | 1:2000 | polyclonal | BIOMOL (GC3008) |
10 | GABA | rabbit | 1:2000 | polyclonal | Sigma (A2052) |
11 | kinesin, heavy chain | mouse | 1:50 | monoclonal | Merck Millipore (MAB1614) |
12 | neurofilament-ilght | mouse | 1:200 | monoclonal | Sigma (N5139) |
13 | GD1a | mouse | 1:10 | monoclonal | Seikagaku Corporation (GMR17) |
14 | GD3 | mouse | 1:10 | monoclonal | Seikagaku Corporation (GMR19) |
15 | GM2 | mouse | 1:10 | monoclonal | Seikagaku Corporation (GMB28) |
16 | GM3 | mouse | 1:10 | monoclonal | Seikagaku Corporation (GMR6) |
17 | VDAC1 | rabbit | 1:40 | polyclonal | Protein Tech (10866-1-AP) |
18 | cytochrome c | mouse | 1:200 | monoclonal | BD Biosciences (556432) |
19 | COX IV | rabbit | 1:100 | monoclonal | Cell Signaling (#4850) |
20 | HSP60 | rabbit | 1:50 | polyclonal | Novus (NB100-91819) |
21 | Parkin | rabbit | 1:100 | polyclonal | Merck Millipore (AB9244) |
22 | PINK1 | rabbit | 1:50 | polyclonal | Novus (NB600-973) |
23 | DJ-1 | rabbit | 1:50 | polyclonal | abcam (ab74268) |
24 | LRRK2 | rabbit | 1:200 | polyclonal | Novus (NB300-268) |
25 | VGAT | guinea pig | 1:500 | polyclonal | Synaptic Systems (131004) |
26 | Rab5B | goat | 1:50 | polyclonal | Santa Cruz Biotechnology (sc-26569) |
Fluorescein conjugated probe | |||||
1 | Alexa 488 conjugated cholera toxin subunit B | 0.5 μg/ml | invitrogen (C-34775) | ||
Fluorescein conjugated secondary antisera | |||||
1 | Alexa 488 conjugated anti-mouse IgG | goat | 1:200 | polyclonal | invitrogen (A-11029) |
2 | Alexa 488 conjugated anti-rabbit IgG | goat | 1:200 | polyclonal | invitrogen (A-11034) |
3 | Alexa 594 conjugated anti-mouse IgG | goat | 1:200 | polyclonal | invitrogen (A-11032) |
4 | Alexa 594 conjugated anti-rabbit IgG | goat | 1:200 | polyclonal | invitrogen (A-11037) |
5 | Alexa 488 conjugated anti-mouse IgM | goat | 1:100 | polyclonal | invitrogen (A-21042) |
6 | Alexa 680 conjugated anti-goat IgG | donkey | 1:100 | polyclonal | invitrogen (A-21084) |
Biotinylated secondary antisera | |||||
1 | biotinylated anti-mouse IgG | horse | 1:200 | polyclonal | Vector (BA-2000) |