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Table 1 Primary and secondary antisera used in this study

From: Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H ß-synuclein

 

Antigen

Host

Dilution

Mono/polyclonal

Source

Primary antisera and antibodies

1

α-synuclein

mouse

1:500 (for EM)

monoclonal

BD Biosciences (610787)

   

1:1000 (for GABA FIHC)

  
   

1:2000

  

2

α-synuclein

rabbit

1:50

polyclonal

Cell Signaling (#2628)

3

phosphorylated α-synuclein

mouse

1:2000

monoclonal

WAKO (pSyn#64)

4

nitrated α-synuclein

mouse

1:50

monoclonal

Santa Cruz Biotechnology (sc-32279)

5

4-hydroxy-2-nonenal

mouse

1:40

monoclonal

NOF Corporation (MHN-020P)

6

Calbindin D-28 k

rabbit

1:5000

polyclonal

Swant (CB38)

7

Parvalbumin

mouse

1:2000

monoclonal

Merck Millipore (MAB1572)

8

Calretinin

rabbit

1:2000

polyclonal

Swant (7699/4)

9

Glutamic acid decarboxylase

rabbit

1:2000

polyclonal

BIOMOL (GC3008)

10

GABA

rabbit

1:2000

polyclonal

Sigma (A2052)

11

kinesin, heavy chain

mouse

1:50

monoclonal

Merck Millipore (MAB1614)

12

neurofilament-ilght

mouse

1:200

monoclonal

Sigma (N5139)

13

GD1a

mouse

1:10

monoclonal

Seikagaku Corporation (GMR17)

14

GD3

mouse

1:10

monoclonal

Seikagaku Corporation (GMR19)

15

GM2

mouse

1:10

monoclonal

Seikagaku Corporation (GMB28)

16

GM3

mouse

1:10

monoclonal

Seikagaku Corporation (GMR6)

17

VDAC1

rabbit

1:40

polyclonal

Protein Tech (10866-1-AP)

18

cytochrome c

mouse

1:200

monoclonal

BD Biosciences (556432)

19

COX IV

rabbit

1:100

monoclonal

Cell Signaling (#4850)

20

HSP60

rabbit

1:50

polyclonal

Novus (NB100-91819)

21

Parkin

rabbit

1:100

polyclonal

Merck Millipore (AB9244)

22

PINK1

rabbit

1:50

polyclonal

Novus (NB600-973)

23

DJ-1

rabbit

1:50

polyclonal

abcam (ab74268)

24

LRRK2

rabbit

1:200

polyclonal

Novus (NB300-268)

25

VGAT

guinea pig

1:500

polyclonal

Synaptic Systems (131004)

26

Rab5B

goat

1:50

polyclonal

Santa Cruz Biotechnology (sc-26569)

Fluorescein conjugated probe

1

Alexa 488 conjugated cholera toxin subunit B

 

0.5 μg/ml

 

invitrogen (C-34775)

Fluorescein conjugated secondary antisera

1

Alexa 488 conjugated anti-mouse IgG

goat

1:200

polyclonal

invitrogen (A-11029)

2

Alexa 488 conjugated anti-rabbit IgG

goat

1:200

polyclonal

invitrogen (A-11034)

3

Alexa 594 conjugated anti-mouse IgG

goat

1:200

polyclonal

invitrogen (A-11032)

4

Alexa 594 conjugated anti-rabbit IgG

goat

1:200

polyclonal

invitrogen (A-11037)

5

Alexa 488 conjugated anti-mouse IgM

goat

1:100

polyclonal

invitrogen (A-21042)

6

Alexa 680 conjugated anti-goat IgG

donkey

1:100

polyclonal

invitrogen (A-21084)

Biotinylated secondary antisera

1

biotinylated anti-mouse IgG

horse

1:200

polyclonal

Vector (BA-2000)