Accumulation of LBs in the postmortem brain of patient PA. (A–C) Immunohistochemical staining of postmortem brain tissue from patient PA. (A) Low magnification image of a midbrain section stained with hematoxylin and eosin (H&E). (A’) High magnification image of the boxed area. Melanin levels were reduced in most of the substantia nigra (SN). (B) (Left) High magnification image of a midbrain section stained with H&E showing the presence of Lewy bodies (LBs) in the SN. (Middle and Right) α-synuclein-positive and pα-synuclein-positive cells in the SN. (C) Confocal microscopy image of a TH (green) and pα-synuclein (red) double-positive SN neuron and a projected merged image: pα-synuclein accumulated in the TH-positive neuron. (D) Melanin levels were reduced in most of the SN. No LBs or α-synuclein-positive neurons were observed in postmortem brain tissue from the father of patient PB. Scale bars: A, 1000 μm; A’, 350 μm; B, C, 50 μm; D, 100 μm.