Figure 1
From: Modulation of medial prefrontal cortical activity using in vivo recordings and optogenetics
Recording and stimulation sites and viral vector expression in the mPFC. (A) Recording sites of pyramidal cells in the infralimbic (IL, n = 11) and prelimbic (PL, n = 12) cortex. (B) Position of the optic fiber in the IL (* indicates tip). (C) Positions of the optic fiber (*) and the recording site (x) in the IL. (A-C) Scale bars, 1 mm. (D) Yellow fluorescent protein (YFP) labeling indicates ChR2 expression restricted largely to IL (deep layers) following rAAV5/CaMKIIa-ChR2(H134R)-EYFP injection into IL. (E) Expression of control vector that lacked the ChR2 sequence (rAAV5/CaMKIIa-EYFP). (D, E) Scale bars, 500 μm. Fm, forceps minor. (F) Example of a ChR2 expressing pyramidal cell in IL. Scale bar, 50 μm. (G-I) Lack of ChR2/viral vector co-expression (green) in GAD positive (GABAergic) interneurons (red). Scale bars, 50 μm. All sections are taken at the level of 3.2 anterior to bregma.