Figure 2
From: Modulation of medial prefrontal cortical activity using in vivo recordings and optogenetics
Effects of optical activation on background activity of individual mPFC neurons. Extracellular single-unit recordings in anesthetized rats. (A) Laser stimulation in IL activated an IL pyramidal cell. (B) Optical stimulation in IL inhibited a PL pyramidal cell. (C) Laser stimulation had no effect on an IL interneuron. Peristimulus time histograms (PSTHs, bin width, 1 s) show number of action potentials (spikes/s) before, during and after laser stimulation (5 mW, 10 Hz). Original recordings of action potentials are shown below PSTHs. Spike 2 software was used to isolate and count single-unit activity based on waveform signal that crossed a trigger level and matched a pre-set shape or template (see Methods). Traces on the right show individual spikes; numbers indicate peak-to-valley spike width.