Figure 5
From: Modulation of medial prefrontal cortical activity using in vivo recordings and optogenetics
Lack of effect of optical activation in animals treated with control virus. Optical stimulation (5 mW, 10 Hz) in the IL of animals injected with a control virus that lacked the ChR2 sequence (rAAV5/CaMKIIa-EYFP) had no effect on background (A) and evoked (B) activity of IL pyramidal cells (n = 5 neurons). (A) Left, Peristimulus time histograms (PSTHs, bin width, 1 s) show number of action potentials (spikes/s) before, during and after laser stimulation in an individual neuron. Right, Bar histograms show summary data (means ± SE) for the sample of neurons tested (n = 5). (B) Left, PSTHs show responses of an individual neuron to innocuous (Innoc.) and noxious (Nox.) stimuli (horizontal bars) before, during and after laser stimulation. Right, Bar histograms show summary data (means ± SE) for the sample of neurons tested (n = 5). Background activity preceding each stimulus has been subtracted from the total activity during stimulation to obtain the net evoked response.