Wild-type Drosophila larvae display stereotyped navigational pattern in response to gentle touch. (A-A”’) Time course of navigational pattern of wild-type 3rd-instar larvae in response to tactile stimuli at anterior segments. “θ” refers to the angle between original direction and reoriented direction of forward movements. The reoriented direction was measured when a larva finished one peristalsis after resuming its forward locomotion. (B) Quantification of larval navigational pattern in response to tactile stimuli. Canton-S (CS) (n=24), Oregon-R (OR) (n=34) and w-1118 larvae (n= 28) showed similar navigational pattern in response to tactile stimulus (7 mN). P>0.05 (one-way ANOVA). (C) Linear regression relationship between the extent of directional changes (°) and the intensity of tactile stimulus (mN). The best-fit line is shown in red. Number of larvae tested: 1 mN, n=28; 3 mN, n=27; 7 mN, n=27; 10 mN, n=26. Error bars represent SEM.