Tutl mutations affected larval navigational pattern in response to gentle touch. (A-A”’) Time course of navigational pattern of tutl23/01085 mutant larvae in response to gentle touch at anterior segments. (B) tutl mutant larvae showed severe defects in adjusting moving direction. The performance of each genotype of tutl mutant larvae was compared to that of wild type. ***p<0.005, t-test. Number of larvae tested: W-1118, n=21; tutl23/23, n=20; tutl23/01085, n=20; tutl23/Df , n=17; tutl01085/Df , n=15. (C) tutl mutant larvae displayed higher numbers of exploratory head swings in response to gentle touch. *p < 0.05, **p < 0.01, ***p<0.005, t-test. (D) tutl mutant larvae took longer time to select a new moving direction after gentle touch. ***p<0.005, t-test. (E) tutl mutant larvae displayed normal withdrawal response after gentle touch. p>0.1, one-way ANOVA test. Error bars represent SEM.