Control of directional change after mechanical stimulation in Drosophila

Table 1 Transgene rescue of the navigational phenotype by expressing a UAS- tutl transgene under control of cell-type-specific GAL4 drivers

GAL4 driver	Expressing pattern	Change in moving direction (°) (Mean±SEM)	Rescue effect
C155-GAL4	All post-mitotic neurons	93.8±5.5	Y^a
Appl-GAL4	Many PNS and CNS neurons	107.0±5.3	Y
Cha-GAL4	Cholinergic neurons in PNS and CNS	74.0±5.2	Y
OK371-GAL4	Glutamatergic neurons (motor neurons and neuronal clusters in the brain)	_^c	N^b
Ddc-GAL4	Dopaminergic and serotonergic neurons	33.4±3.5	N
RN2-GAL4	RP2, aCC and pCC	38.4±3.7	N
G11-1-GAL4	Embryonic PNS	49.6±5.6	N
ftz.ng-GAL4	Subsets of neurons	40.0±4.4	N
D42-GAL4	Motor neurons and PNS neurons	76.4±8.7	N
TrpA1-GAL4	CNS neurons expressing TrpA1 gene	51.1±6.9	N
5-HTR1B-GAL4	Neurons expressing serotonin receptor 1B	46.9±4.5	N
C81-GAL4	CNS neurons with diffuse expression throughout brain lobes	45.8±4.7	N
SN(5-40)-GAL4	All sensory neurons	56.6±5.1	N
NompC-GAL4	Class I , bd neurons and chordotonal organs	47.7±4.0	N
iav-GAL4	Chordotonal organs	46.2±3.5	N
Pain-GAL4	md neurons, chordotonal organs and some CNS neurons	52.1±4.7	N
ppk1.9-GAL4	Class IV da neurons	51.2±5.3	N

3^rd-instar tutl²³/tutl²³ mutant larvae, in which UAS-tutl was under control of different cell-type-specific GAL4 drivers, were examined for navigational pattern in response to tactile stimuli. Their performance was compared to that of tutl²³/tutl²³ mutant larvae that only carry GAL4.
^a “Y” indicates significant rescue (p<0.05).
^b “N” indicates no significant rescue.
^c Expression of tutl under control of Ok371-GAL4 in tutl mutant larvae caused a failure for larvae to resume forward movement after gentle touch.

ISSN: 1756-6606