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Figure 3 | Molecular Brain

Figure 3

From: Mind bomb-1 is an essential modulator of long-term memory and synaptic plasticity via the Notch signaling pathway

Figure 3

Normal structural integrity of mib1 cKO brains. (A) Hematoxylin & Eosin staining of paraffin-embedded sections of 6-month-old wild-type (left panel) and mib1 cKO (right panel) hippocampi. Scale bar: 200 μm. (B) MAP2 (upper panels) and GFAP (lower panels) immunoreactivity in the CA1 regions of 6-month-old wild-type (left panels) and mib1 cKO (right panels) hippocampi. py, pyramidal neuron layer; s.r., stratum radiatum; s.l.m., stratum lacunosum molecular. Scale bars: 50 μm. (C) Synaptophysin immunoreactivity in the CA1 regions of 6-month-old wild-type (left panel) and mib1 cKO (right panel) hippocampi. py, pyramidal neuron layer; s.r. stratum radiatum. Scale bar: 50 μm. (D) Representative confocal images of neurobiotin-labeled pyramidal neurons in the CA1 regions of 3-month-old wild-type and mib1 cKO hippocampi (left panel). Spine density in CA1 pyramidal neurons was expressed as spines per 10-μm length on secondary dendrites that were located 150–200 μm away from the cell body (WT, n = 9, N = 6; cKO, n = 11, N = 7) (right panel). The number of cells (n) and mice (N) used in each experiment is indicated. Scale bar: 2 μm. Error bars show standard deviation.

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