Figure 1
From: Activation of GABAB receptors inhibits protein kinase B /Glycogen Synthase Kinase 3 signaling
Activation of GABA B receptors elevates GSK-3α/β phosphorylation at Ser21/Ser 9 sites, but has no effect on phosphorylation of GSK-3α/β at Y279/Y216 sites in HEK293T cells expressing GABA B receptors. A. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) levels in extract prepared from HEK293T cells transfected with GABAB receptor in the presence of GABAB receptor antagonist and/or agonist. GSK-α/β was used as a loading control. B. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). The intensity of phospho-GSK-3α/β was quantified by densitometry (software: Image J, NIH). C. Western blot analysis of phosphorylated GSK-3α/β (Y-279/Y-216) levels in extract prepared from HEK293T cells transfected with GABAB receptor in the presence of GABAB receptor antagonist and/or agonist. GSK-3α/β was used as a loading control. D. Densito-metric analysis of phosphorylated GSK-3α/β (Y-279/Y-216). The intensity of phospho-GSK-3α/β was quantified by densitometry (software: Image J, NIH). Data were analyzed by one-way ANOVA (*P < 0.05, **P < 0.01, n = 3).