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Figure 2 | Molecular Brain

Figure 2

From: Activation of GABAB receptors inhibits protein kinase B /Glycogen Synthase Kinase 3 signaling

Figure 2

Activation of GABA B receptors elevates Akt (Thr-308) phosphorylation in HEK293T cells expressing GABA B receptors, which can be blocked with pretreatment of PI3K inhibitor Wortmannin. A. Western blot analysis of phosphorylated Akt (Thr-308) or Akt (Ser-473) levels in extract prepared from HEK 293T cells transfected with GABAB receptor in the presence of GABAB receptor antagonist and/or agonist. Akt was used as a loading control. B-C. Densitometric analysis of phosphorylated Akt (Thr-308) (B) and Akt (Ser-473) (C). The intensity of phospho-Akt was quantified by densitometry (software: Image J, NIH). D. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) or GSK-3α/β levels in extract prepared from HEK293T cells transfected with GABAB receptor pretreated with/without Wortmannin (100 nM, 24 h) in the presence or absence of GABAB receptor agonist SKF97541. E. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). The intensity of phosphor- GSK-3α/β (Ser-21/Ser-9) was quantified by densitometry (software: Image J, NIH). Data were analyzed by one-way or two-way ANOVA(*P < 0.05, **P < 0.01, n = 3).

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