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Figure 3 | Molecular Brain

Figure 3

From: Activation of GABAB receptors inhibits protein kinase B /Glycogen Synthase Kinase 3 signaling

Figure 3

Effect of GABA B receptors on GSK-3α/β phosphorylation is a Gi protein-independent/β-arrestin2-dependent pathway in HEK293T cells expressing GABA B receptors. A. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) levels in extract prepared from HEK293T cells transfected with GABAB receptors in the presence or absence of GABAB receptor agonist with/without PTX (200 ng/ml, 14–18 h). GSK-3α/β was used as a loading control. B. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). C. Western blot analysis of efficacy of β-arrestin2 siRNA on β-arrestin2 express in HEK293T cells. D. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) levels in extract prepared from HEK293T cells transfected with GABAB receptors and β-arrestin2 siRNA or control siRNA in the presence or absence of GABAB receptor agonist. GSK-3α/β was used as a loading control. E. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). The intensity of phospho-GSK-3α/β was quantified by densitometry (software: Image J, NIH). Data were analyzed by two-way ANOVA(*P < 0.05, **P < 0.01, n = 3).

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