Figure 7

Impaired spatial learning and fear memory in the 1d2d-PSD-95-EGFP KI mice. A–C, Barnes circular maze (WT, open circles, n = 15; KI, filled circles, n = 16): latency to reach the target hole (A) and number of errors before reaching the target hole (B) across the training sessions were counted. Time spent around each hole in the probe test conducted after 24 trials, as shown in A and B was recorded (C). D–E, Eight-arm radial maze (WT controls, open circles, n = 15; KI, filled circles, n = 12): KI mice showed low levels of different arm choices in the first 8 entries (D), and increased revisiting errors (E) during training. F–N, Contextual fear conditioning test. In the fear conditioning phase, KI mice (filled circles, n = 12) showed higher freezing responses than WT controls (open circles, n = 15) (F). Horizontal bars denote exposure to the conditioning stimulus (tone), and arrows indicate the timing of the unconditioned stimulus (footshock). Freezing behaviors and distance traveled in the context test at 24 h after fear conditioning (G, K). Freezing behaviors and distance traveled in the cued test with an altered context at 24 h after fear conditioning (H, L). Freezing behaviors and distance traveled in the context test at 7 days after fear conditioning (I, M). Freezing behaviors and distance traveled in the cued test with altered context at 7 days after fear conditioning (J, N).