Trace fear memory is mediated by postsynaptic CP-AMPAR trafficking within the ACC. (A) (Left) We performed whole cell patch clamp recordings immediately following trace fear conditioning in ACC slices from transgenic FosGFP mice. (Right) We recorded EPSCs from pyramidal neurons in layer II/III whilst stimulating layers V/VI of the ACC. (B) Trace fear conditioning induces c-fos activity within the ACC; scale bar represents 100 μm. (C) Representative images of whole-cell patch clamp recordings of FosGFP negative pyramidal neurons (top panels), and FosGFP positive pyramidal neurons (bottom panels) as indicated by yellow showing overlap between GFP and dye loaded pipette; scale bar represents 20 μm. (D) Representative traces of single pyramidal neuron recordings before "a" and after "b" NASPM application. (E-F) NASPM significantly inhibited the amplitude of FosGFP positive neuronal EPSCs to 67% of the baseline, a reduction that is significantly greater than that observed on FosGFP negative neurons (FosGFP positive: n = 6; FosGFP negative: n = 9, t = -2.38).