Figure 3

iDG phenotype and severe working memory deficit in SNAP-25 KI mice. (A–C) Scatter plot illustrating the fold change (FC) values for gene expression levels in the hippocampi of SNAP-25 KI mice (control: n = 7 mice, KI: n = 6 mice) and FLX-treated (A), Shn-2 KO (B), or αCaMKII HKO mice (C), which have the iDG phenotype. (D) mRNA expression analysis of DRD1A, DSP, and TDO2, which identify the iDG phenotype, using real-time PCR (control: n = 7 mice, KI: n = 9 mice). (E, F) The somatic physiological properties of granule cells in the DG of control (n = 22) and SNAP-25 KI mice (n = 21) are shown. (E) Sample recordings of granule cell action potentials evoked by depolarizing current pulses are shown. (F) Pooled data showing the resting membrane potential, input resistance, spike threshold current, and maximal number of spikes during sustained depolarization. (G) EPSP-to-fiber volley ratio (control: n = 9, KI: n = 8), synaptic potentiation induced by 10 μM dopamine (control: n = 5, KI: n = 4), and the frequency facilitation induced by a 1-Hz stimulation (control: n = 8, KI: n = 7) at the mossy fiber synapse are shown. (H) Severe working memory deficits were observed in SNAP-25 KI mice during a spontaneous alternation task in the T-maze (control: n = 9 mice, KI: n = 7 mice).