Altered cortical interneuron laminar distribution in Disc1- L100P adult mice. Quantification of both CB- and PV-labeled cells was achieved by counting the number of fluorescent cells in each octant and expressed as a percentage of total number per ROI of fixed size across the neocortex. (A) There was a significant interaction effect with CB-interneurons across the octants between WT and Disc1- L100P mice (two-way ANOVA, p < 0.01) with proportionally more CB-interneurons in the middle cortical layers of octants 3 and 4 (t-test, p < 0.01). Conversely, there were fewer CB+ cells in the second, sixth and seventh octants in the Disc1- L100P mutant versus WT (n = 52 ROIs from 4 different mice per group; t-test, p < 0.01). (B) PV-labeled interneurons were significantly different in their distribution across the octants between WT and Disc1- L100P mutants (two-way ANOVA, p < 0.01). More PV + cells were observed in WT of octant 6 but less in superficial cortical layers of octant 1 than in the mutants (n = 84–90 ROIs from 5 different mice per group; t-test, p < 0.01). Scale Bar, 300 μm. All data are shown as mean ± SEM; * p < 0.05, ** p < 0.01 versus WT. CB, calbindin; PV, parvalbumin; ROI, region of interest.