Selective increase of PV-interneurons in CA1 and CA2/3 regions of the hippocampus in Disc1- L100P adult mutant mice. (A) PV-immunostained images of the hippocampus in WT and Disc1- L100P mice were divided into CA1, CA2/3 and DG subfields in which labeled cells were counted. Scale Bar, 300 μm. A higher magnification of each subfield is shown on the right. Scale Bar, 100 μm. (B) Disc1- L100P mutants had significantly more PV-interneurons in the hippocampus, particularly within CA1 and CA2/3 subfields when compared to WT (n = 12 ROIs for each region from 4 different mice per group). (C) There was no significant difference in the number of CB+ interneurons within the CA1 and CA2/3 regions between WT and Disc1- L100P mutants. DG was excluded from analysis due to the absence of CB-interneurons. All data are shown as mean ± SEM; ** p < 0.01 versus WT. CB, calbindin; PV, parvalbumin; CA, Cornu Ammonis; DG, dentate gyrus.