Figure 1
From: Regulation of epileptiform activity by two distinct subtypes of extrasynaptic GABAA receptors
Tonic GABA current increased after the overexpression of α5β3γ2 GABA A receptors. A, Typical GABA (100 μM) induced currents in HEK293T cells transfected with α5β3γ2 subunits (left panel), which could be largely blocked by α5 subunit-specific inverse agonist L655,708 (100 nM, right panel). B, Summarized data showing GABA-induced α5β3γ2 receptor currents in HEK293T cells significantly inhibited by L655,708 (Control, 437.1 ± 63.1 pA, n = 10; L655,708, 142.8 ± 25.7 pA, n = 10; ***, p < 0.001). C, Typical GABA current traces in cultured hippocampal neurons transfected with mCherry or plus the α5β3γ2 subunits. D, Bar graphs showing no significant difference between the total whole-cell GABA currents in neurons transfected with mCherry or plus the α5β3γ2 subunits. E, Representative tonic GABA currents revealed by rapid application of GABAA-R blocker bicuculline (100 μM) in hippocampal neurons transfected with mCherry or plus the α5β3γ2 subunits. F, Summarized data showing that tonic GABA current in α5β3γ2-transfected neurons (24.5 ± 4.2 pA, n = 8) was significantly increased in comparison with the control neurons (13.1 ± 1.7 pA, n = 8; *, p < 0.05).