Inhibition of epileptiform activity in cultured hippocampal neurons overexpressing α5β3γ2 receptors. A, Typical traces from two hippocampal neurons showing the epileptiform burst activity after chronic CTZ treatment (5 μΜ, 24 h). Panel b shows the expanded view of a single epileptiform burst from panel a. Epileptiform burst is characterized by a train of action potentials on a large depolarization shift. B, Representative traces showing the lack of epileptiform bursts in two hippocampal neurons transfected with the α5β3γ2 receptors. C, Bar graphs illustrating that overexpression of α5β3γ2 receptors significantly reduced the percentage of neurons showing epileptiform activity after chronic CTZ treatment (mCherry, ~90%, n = 29; α5β3γ2, ~33%, n = 33; ***, p < 0.001). D, Neurons transfected with α5β3γ2 receptors showing lower burst frequency (0.49 ± 0.16 per min, n = 33), compared to mCherry controls after CTZ treatment (1.83 ± 0.35 per min, n = 29; ***, p < 0.001).