Figure 4
From: Regulation of epileptiform activity by two distinct subtypes of extrasynaptic GABAA receptors
Overexpression of α6β3δ receptors inhibits epileptiform bursting activity in cultured hippocampal neurons. Aa, Representative traces showing the typical recurrent epileptiform bursts after chronic pretreatment with CTZ (5 μM, 24 h) in two different GFP-transfected pyramidal neurons. Ab, A single epileptiform burst in (a) was expanded to show a train of action potentials overlaying on a large depolarization shift. Ba, Representative traces showing the lack of typical epileptiform bursts in two α6β3δ-transfected hippocampal neurons. Bb, Expanded view of the boxed activity in (a). C, Bar graph showing the percentage of neurons with epileptiform bursting activity after chronic treatment with CTZ (5 μM for 24 h). *** p < 0.001, Pearson Chi-Square test. D, Bar graph showing a significant reduction of the average epileptiform burst frequency in neurons transfected with α6β3δ receptors, comparing to GFP controls after CTZ treatment. ** p < 0.01.