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Figure 3 | Molecular Brain

Figure 3

From: Dock3 interaction with a glutamate-receptor NR2D subunit protects neurons from excitotoxicity

Figure 3

Identification of Dock3 as an NR2D-interacting molecule. (A) Schematic diagram of NR2D and a probe used for yeast two-hybrid screening. M1–M4 indicates the transmembrane regions. The numbers refer to the amino-acid positions of NR2D. (B) Schematic diagram of Dock3. The selected interaction domain (SID) corresponds to the minimal region common to all fragments identified by yeast two-hybrid screening. The numbers indicate the amino-acid positions within Dock3. DHR-1, Dock homology region 1; DHR-2, dock homology region 2; PRR, proline-rich region. (C) The interaction between NR2D and Dock3 in HEK293T cells. HEK293T cells were transfected with plasmids encoding the SID of Dock3 (Myc-Dock3 SID) and EGFP-tagged NR2D C-terminus (EGFP-NR2D CT). Lysates of transfected cells were immunoprecipitated (IP) with anti-GFP (left panel) or anti-Myc antibodies (right panel). Immune complexes were detected by Western blotting with anti-GFP or anti-Myc antibodies. In lanes 1–3, 1/10th volumes of the lysates used for immunoprecipitation were loaded for TCL samples. IB, Immunoblotting.

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