Figure 3
From: An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch
Neuroligin-2 regulates GABA equilibrium potential. A, Representative current clamp recordings showing differential changes in membrane potentials evoked by GABA in 10–13 DIV control neurons (gray) and NL2shRNA-transfected neurons (black). Note that GABA evoked action potentials in most NL2shRNA-transfected neurons (4 out of 5 neurons) but rarely in control neurons (1/6). B, Representative voltage-clamp recordings showing GABA-evoked currents at different holding potentials in the presence of TTX (0.5 μM) and DNQX (10 μM). C, I-V plot of the mean GABA-evoked peak currents in control neurons and neurons transfected with NLmiR, NL2shRNA, or NL2. Note the opposite shift in EGABA between NL2 overexpression and NL2 knockdown groups. D, Bar graphs showing mean EGABA of control neurons and neurons transfected with NLmiR, NL2shRNA, or NL2. Dashed line indicates the mean EGABA of control neurons. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA.