Figure 7
From: An unexpected role of neuroligin-2 in regulating KCC2 and GABA functional switch
Neuroligin-2 regulates dendritic spines through KCC2. A-C, Representative images showing dendritic spines in neurons (15 DIV) transfected with GFP (A), GFP-NL2shRNA (B), or GFP-NL2shRNA + KCC2 (C). Scale bar, 20 μm. D, Enlarged inlets in A-C showing dendritic spines (green) overlaid with glutamatergic presynaptic terminals labeled by vGlut1 (red). Scale bar, 5 μm. E, Quantification of dendritic spine numbers per 20 μm in neurons expressing GFP, GFP-NL2shRNA, or GFP-NL2shRNA + KCC2. n = 11 per group. F, Bar graphs showing somatic KCC2 immunofluorescence intensity: control neurons, 153 ± 9, n = 17; GFP-NL2shRNA transfected neurons, 88 ± 14, n = 11; GFP-NL2shRNA + KCC2 transfected neurons, 168 ± 13, n = 9. a.u., arbitrary unit. G, Bar graphs showing the frequency of mIPSCs (n = 9 per group; left Y-axis) and mEPSCs (n = 11 per group; right Y-axis). mIPSCs: Control, 0.58 ± 0.14 Hz; GFP-NL2shRNA, 0.02 ± 0.01 Hz; GFP-NL2shRNA + KCC2, 0.03 ± 0.02 Hz. mEPSCs: Control, 2.55 ± 0.42 Hz; GFP-NL2shRNA, 0.12 ± 0.03 Hz; GFP-NL2shRNA + KCC2, 1.07 ± 0.22 Hz. *p < 0.05, ***p < 0.001, n.s., not significant, one-way ANOVA.