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Figure 3 | Molecular Brain

Figure 3

From: Acute 5-HT7 receptor activation increases NMDA-evoked currents and differentially alters NMDA receptor subunit phosphorylation and trafficking in hippocampal neurons

Figure 3

5-HT7 receptor activation increases NR1 subunit phosphorylation. A) Hippocampal slices were treated with 300 nM LP 12 in the absence or presence of 1 μM SB 258719 for 5 min. Increases in the phosphorylation state at serine 897 were normalized to control (vehicle-treated slices) and to total NR1. Data represent the average and standard error of 9 independent experiments. ** p < 0.01 LP 12 vs. vehicle, # p < 0.01 vs. LP 12 vs. LP 12 + SB 258719, * p < 0.5 compared to vehicle, ANOVA analysis with Bonferroni’s post-test. B) Hippocampal slices were treated as in A and western blots were performed using an anti-phospho-serine 896 antibody. Data represent the average and standard error of 10 independent experiments. ** p < 0.01 LP 12 vs. vehicle, # p < 0.01 vs. LP 12 vs. LP 12 + SB 258719, ANOVA analysis with Bonferroni’s post-test. C) Representative Western blots showing serine 896 and serine 897 phosphorylation, total NR1, and β-actin.

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