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Table 1 5-CT treatment differentially alters NMDA receptor subunit phosphorylation

From: Acute 5-HT7 receptor activation increases NMDA-evoked currents and differentially alters NMDA receptor subunit phosphorylation and trafficking in hippocampal neurons

NMDAR subunit/phosphorylation site

Treatment (5 min)

 

5-CT

5-CT + SB

SB 269970

NR1/896, n = 8

2.07 ± 0.39**

0.91 ± 0.31

1.06 ± 0.28

NR1/897, n = 8

1.51 ± 0.13**

1.00 ± 0.39

0.95 ± 0.11

NR2A/total, n = 10

0.71 ± 0.07**

0.97 ± 0.37

0.86 ± 0.17

NR2B/1472, n = 5

2.79 ± 0.37**

1.49 ± 0.61

2.86 ± 1.33

NR2B/1252, n = 10

1.39 ± 0.49

1.50 ± 0.23

1.53 ± 0.31

NR2B/1336, n = 7

1.25 ± 0.19

1.98 ± 0.31

2.36 ± 0.36**

  1. Hippocampal slices were treated with 50 nM 5-CT in the absence or presence of 1 μM SB 269970 for 5 min. Changes in the phosphorylation state for each antibody were normalized to control (vehicle-treated slices) and to total NR subunit expression. For each experiment a β-actin antibody was used to confirm equal loading. Data presented are the average and standard error. * p < 0.5, ** p < 0.01, compared to vehicle.
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Molecular Brain

ISSN: 1756-6606