Figure 1

Phenotypic analysis of adult Dll1^(loxP/loxP)/hGFAP-Cre mice. (A) RT-PCR analysis shows the Cre-mediated deletion of Dll1 in cultured astrocytes from Dll1^(loxP/loxP)/hGFAP-Cre mice cerebella. (B, C) Hematoxylin and eosin stained sagittal sections from hGFAP-Cre (B) and Dll1^(loxP/loxP)/hGFAP-Cre (C) mice. Boxed areas are shown as higher magnifications in insets in (B) and (C). (D, E) Granule cells labeled by an anti-NeuN antibody (red) in hGFAP-Cre (D) and Dll1^(loxP/loxP)/hGFAP-Cre (E) mice. (F, G) Purkinje cells labeled by an anti-calbindin antibody (red) in hGFAP-Cre (F) and Dll1^(loxP/loxP)/hGFAP-Cre (G) mice. (H, I) BG labeled by anti-GFAP (green) and anti-β-galactosidase (red) antibodies in hGFAP-Cre (H) and Dll1^(loxP/loxP)/hGFAP-Cre (I) mice. Scale Bars: 1 mm (B, C), 50 μm (Insets in B and C), 100 μm (D, E), 50 μm (F-I). IGL, internal granular layer; ML, molecular layer; PCL, Purkinje cell layer.