Figure 10
Biochemical analysis of protein Tau in GSK3α deficient mice. A. Biochemical analysis by western blotting of total protein extracts from forebrain of AAC and AA- mice aged 3, 6 and 18 months (n=5/age). Tau protein levels are normalized to actin and expressed relative to control AA- mice at age 3 months. Data (mean±SEM) are statistically analyzed by two-way Anova (Bonferroni post hoc test), genotype: F(1,24)=3.40, p=0.5093; age: F(2,24)=37.60, p=0.0027; interaction: F(2,24)=0.20, p=0.7759. Lower panels show representative western blots. B. Biochemical analysis by western blotting for phospho-epitopes pS396/404, pS199 and pT231 of endogenous mouse Tau in total protein extracts from hippocampus and forebrain of AAC and control AA- mice. C. Biochemical analysis by western blotting for phospho-epitopes pS396/404, pS199 and pT231 of endogenous mouse Tau in total protein extracts from hippocampus and cortex of GSK3α.KO and FVB wild-type mice. D. Biochemical analysis by western blotting for phospho-epitopes pS396/404, pS199 and pT231 of human Tau.P301L in total protein extracts from hippocampus and cortex of GSK3α.KOxTau.P301L mice and the parental Tau.P301L mice. In panels B-D, all data are normalized for total Tau and reported relative to the respective control mice. Data (mean±SEM) are statistically analyzed by unpaired Student’s t-test (two-tailed), n=6 or 7 per genotype; * p<0.05.