Figure 7
Electrophysiology of neuron-specific GSK3α deficient mice. A. Input–output curve generated by gradually increasing stimulus intensity in sections from AAC mice compared to control AA- mice (n=6). Two-way Anova; genotype: F(1,399)=15.20, *** p<0.0001; stimulation intensity: F(6,399)=106.9, p<0.0001; interaction: F(6,399)=1.256, p=0.2766. B. Paired pulse facilitation (PPF) induced in 4 intervals of 25, 50, 100 and 200 ms in CA1 stratum radiatum of sections from AAC mice compared to AA- mice (n=6). Data are expressed as the ratio of the second to the first fEPSP slope. Two-way Anova; genotype: F(1,220)=1.124, p=0.2903; time: F(3,220)=34.35, p<0.0001; interaction: F(3,220)=1.685, p=0.1710. C. Synaptic fatigue analyzed for 1 to 10 stimulations in sections from AAC mice compared to AA- mice (n=6). Two-way Anova; genotype: F(1,320)=67.23, **** p<0.0001; stimulus: F(9,320)=321.3, p<0.0001; Interaction: F(9,320)=1.030, p=0.4153. D. LTP was recorded in CA1 stratum radiatum in sections from AAC and AA- mice (n=6). L-LTP was analyzed during the last 2 hours of recording. Unpaired Student’s t-test (two-tailed); *p=0.0369.