Figure 8
Biochemical repercussions of PIA on GSK3 and protein tau in hippocampus of wild-type mice. A. Biochemical analysis of GSK3 by western blotting of total protein extracts from hippocampus of FvB wild-type mice (n=10) at different timepoints before or after they performed the PIA task (n=6 per timepoint). All data normalized to actin and reported relative to naive control mice. Data (mean±SEM) are statistically analyzed by one-way Anova (Dunnet’s post hoc test compared to naive). GSK3α: F(3,24)=0.5422; p=0.6581. GSK3β: F(3,24)=1.523; p=0.2339. GSK3α pS21: F(3,24)=12.58; p<0.0001. GSK3α pS21/total: F(3,24)=12.11; p<0.0001. GSK3β pS9: F(3,24)=5.240; p=0.0063. GSK3β pS9/total: F(3,24)=4.832; p=0.0090. GSK3α pY279: F(3,24)=4.973; p=0.0080. GSK3α pY279/total: F(3,24)=3.346; p=0.0359. GSK3β pY216: F(3,24)=3.875; p=0.0216. GSK3β pY216/total: F(3,24)=4.528; p=0.0119. * p<0.05; ** p<0.01; *** p<0.001. Lower panels show representative western blots. B. Biochemical analysis of protein Tau by western blotting of total protein extracts from hippocampus of FvB wild-type mice (n=10) at different timepoints before or after they performed the PIA task (n=6 per timepoint). All data normalized to actin and reported relative to naive control mice. Data (mean±SEM) are statistically analyzed by one-way Anova (Dunnet’s post hoc test compared to naive). Tau5: F(3,24)=7.625; p=0.0009. AD2: F(3,24)=5.162; p=0.0068. AD2/Tau5: F(3,24)=1.761; p=0.1815. * p<0.05; ** p<0.01; *** p<0.001. Lower panels show representative western blots.