Figure 9
Biochemical repercussions of NORT on GSK3 and Tau in hippocampus of wild-type mice. A. Biochemical analysis of GSK3 by western blotting of total protein extracts from hippocampus of FvB wild-type mice (n=10) at difference timepoints before or after they performed the NORT task (n=6 per timepoint). All data normalized to actin and reported relative to naive control mice. Data (mean±SEM) are statistically analyzed by one-way Anova (Dunnet’s post hoc test compared to naive). GSK3α: F(3,24)= 0.6609; p=0.5842. GSK3β: F(3,24)= 1.131; p=0.3566. GSK3α pS21: F(3,24)= 1.351; p=0.2816. GSK3α pS21/total: F(3,24)= 1.529; p=0.2327. GSK3β pS9: F(3,24)= 1.469; p=0.2479. GSK3β pS9/total: F(3,24)= 1.699; p=0.1939. GSK3α pY279: F(3,24)= 1.002; p=0.4091. GSK3α pY279/total: F(3,24)= 1.871; p=0.1614. GSK3β pY216: F(3,24)= 1.302; p=0.2967. GSK3β pY216/total: F(3,24)= 1.580; p=0.2203. Lower panels show representative western blots. B. Biochemical analysis of protein Tau by western blotting of total protein extracts from hippocampus of FvB wild-type mice (n=10) at difference timepoints before or after they performed the NORT task (n=6 per timepoint). All data normalized to actin and reported relative to naive control mice. Data (mean±SEM) are statistically analyzed by one-way Anova (Dunnet’s post hoc test compared to naive). Tau5: F(3,24)= 3.286; p=0.0380. AD2: F(3,24)= 1.637; p=0.2071. AD2/Tau5: F(3,24)= 2.593; p=0.0761. * p<0.05. Lower panels show representative western blots.