Figure 5

Appearance of Iba-1⁺/CD45⁺round cells in the LPS-injected SNpc. (A, C, D) Serial sections were obtained at the indicated times after LPS injection and labeled with Iba-1 (A), CD45 (C), and Iba-1/CD45 (D) antibodies. (A, C) Lower panels are higher magnification images of the boxed areas in the upper panels. (A) In intact contralateral (Contra) sides and PBS-injected brains, Iba-1⁺ cells were ramified (black arrows). In LPS-injected brain, round Iba-1⁺ cells were detectable at 1 d (white arrowheads), and the number of these cells increased at 3 d but decreased at 7 d. In SNr areas, Iba-1⁺ cells became ramified and highly expressed Iba-1 at 7 d (white arrows). (B) Iba-1⁺ cells at the indicated times after LPS injection were quantified. Values are means ± SEMs of more than three samples (*p < 0.001; Student’s t-test). (C) CD45⁺ cells were detectable at 1 d (white arrowheads), and the number of these cells increased at 3 d but decreased at 7 d. Some round CD45⁺ cells were found around blood vessels (BV) (inset ‘a’ in 3 d). (D) Brain sections obtained at 3 d were double-labeled with Iba-1 and CD45 antibodies, and visualized with Alexa Fluor 488- and 555-conjugated antibodies, respectively. Asterisks (*) indicate drug injection sites. Scale bars, 200 μm (upper panels in A, C), 50 μm (lower panels and the inset in A, C), and 20 μm (D).