In vitro characterization of the NS/PCs derived from transgenic mice that ubiquitously express ffLuc-cp156. A, Fluorescence image showing NS/PCs derived from fetal transgenic mice expressing the fluorescent protein cp156-Venus, which is originally modified from GFP. B, Bioluminescent signals originating from the luciferase were detected in the NS/PCs by BLI. C, The number of NS/PCs was significantly correlated with the measured photon count by BLI. Values are means ± SEM (n = 3). D, Tertiary NS/PCs differentiated into Tuj-1+ neurons, GFAP+ astrocytes, and CNPase+ oligodendrocytes in vitro. E, Quantitative analysis of Tuj-1+ neurons, GFAP+ astrocytes, and CNPase+ oligodendrocytes in vitro. Values are means ± SEM (n = 5). Scale bar: 100 μm in (A) and 50 μm in (D).