Distribution of grafted cells in the sub-acute and chronic TP groups. A, Representative images of GFP-immunostained sagittal sections in the sub-acute and chronic TP groups 42 days after transplantation. B, Higher-magnification images of the boxed areas in (A). C, Quantitative analysis of the GFP+ grafted cell area in axial sections 42 days after transplantation. Grafted cells were located at the epicenter, rostral, and caudal sites in the sub-acute TP group, whereas they were limited to the lesion epicenter in the chronic TP group. Values are means ± SEM (n = 4). *P < 0.05. D, Representative images of GFP- and CS56-immunostained sagittal sections from the sub-acute and chronic TP groups. E, Higher-magnification images of the boxed area in (D). In the sub-acute TP group, the GFP+ grafted cells migrated away from the graft site due to less accumulation of CS56+ CSPG around the lesion site, while the robust CSPGs prevented further migration of the grafted cells in the chronic TP group. Scale bar: 1000 μm in (A), 100 μm in (B), 500 μm in (D) and 200 μm in (E).