Figure 5

Comparison of the effects of NS/PC transplantation between the sub-acute and chronic TP groups. A, Representative HE- and LFB-stained images of axial sections at the lesion epicenter in the sub-acute TP, chronic TP, and both control groups. B, Significant atrophy of the spinal cords was observed in the chronic TP and control groups compared to that of the sub-acute TP group. Values are means ± SEM (n = 3). ^*P < 0.05. C, The sub-acute TP group demonstrated a significantly larger myelinated area than the chronic TP and control groups. Values are means ± SEM (n = 3). ^*P < 0.05. D, Representative images of sagittal sections stained for NF-H at the rim of the lesion epicenter and of axial sections stained for 5HT at the lumbar intumescence in the sub-acute TP, chronic TP, and each control group. E, Greater area of NF-H⁺ neuronal fibers was observed in the sub-acute TP group than in the chronic TP group or control groups. Values are means ± SEM (n = 3). ^*P < 0.05, ^**P < 0.01. F, Significantly larger areas of 5HT⁺ serotonergic fibers were detected in the sub-acute TP group than in the chronic TP or control groups. Values are means ± SEM (n = 3). ^**P < 0.01. Scale bar: 100 μm in (A) and (D).