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Figure 3 | Molecular Brain

Figure 3

From: The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis

Figure 3

NEAT1_2 lncRNA is not expressed in the nuclei of WT mouse spinal motor neurons. A. The expression levels of total NEAT1, NEAT1_1 and NEAT1_2 transcripts in tissues of 8-week-old mice are displayed using quantitative RT-PCR. Data represent mean ± s.d. (n = 3). B. NEAT1_2 nuclear lncRNA does not appear in the control mouse spinal cord. (a-c) a motor neuron in 8-week-old mouse spinal cord. (d-f) a motor neuron in 2-y-old mouse spinal cord. The motor neurons in the ventral horn were distinguished from glial cells by their morphological features. Dotted lines represent the outline of the nucleus. Arrowheads: colon epithelial cells served as a positive control for NEAT1_2 detected by in situ hybridization (g, h). Lipofuscin in the cytoplasm, which is easily formed in aged motor neurons and has autofluorescence, is denoted as an asterisk (d, f). A long-path filter was used to distinguish Hoechst from autofluorescence. Scale bars, 10 μm.

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Molecular Brain

ISSN: 1756-6606