Figure 3
Enhancement or inhibition of GABA A Rs with subunit-selective drugs reveals no difference in single-channel current amplitude. (A and B) Plots of current variance against mean tonic GABAAR currents in wild-type (WT) cells in the absence (filled circles) and presence (empty circles) of 300 nM THIP (A), and 100 μM furosemide (B, fur). (C and D) Same as (A and B), but for Ts65Dn GCs (filled and empty triangles). A single least-squares line is fitted to values in the absence and presence of each drug in each plot, as there were no difference between the slopes and y-intercepts of lines fitted to values in the absence or presence of each drug in either wild-type or Ts65Dn GCs (p > 0.05 for all comparisons, ANCOVA). The slopes and intercepts of the lines shown did not differ between wild-type and Ts65Dn GCs (slope and intercept: THIP, p = 0.8783 and 0.8539; furosemide, p = 0.936 and 0.06818; ANCOVA). The single-channel current, i, calculated as the slope of each line, is shown on each plot.