Expression of NMDA receptor subunits in mouse retinal ganglion cell. (A-C) Immunohistochemical analysis of Brn3 (B red) in Thy1-CFP mice. CFP fluorescence (A green) was overlaid with Brn3 (C). Arrows in (C) indicate double-labeled cells. Scale bar, 20 μm. (D-E) After dissociation the fluorescent RGC was picked up from the cell suspension. CFP (green) and DIC pictures for the same isolated cell are superimposed (E). Arrowhead indicates CFP-expressing RGC. Scale bar, 20 μm. (F) Single-cell RT-PCR analysis for GluN1, four GluN2 subunits, Brn3 and β-actin. Distilled water was used for PCR negative control. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RGC, retinal ganglion cell.