Expression of the two major proteins for dopamine metabolism and α- synuclein in the striata of wild-type and Sept4 transgenic mice. (A) The nigrostriatal tract (parasagittal sections) of Sept4+/+ and Sept4Tg/+ mice immunostained for the dopamine transporter (DAT). There was no recognizable morphological alteration in the nigrostriatal tract of Sept4Tg/+ mice. (B–D) Quantitative immunoblot analysis for DAT, tyrosine hydroxylase (TH), α-synuclein (α-Syn), and α-Syn phosphorylated at Ser132 (p-α-Syn) in the fractionated lysate of the striata of Sept4+/+ and Sept4Tg/+ mice. There were no significant quantitative changes in these proteins by the chronic overload of SEPT4.